J. THOMAS McCLINTOCK, Ph.D.

DNA Diagnostics, Inc.
P.O. Box 4544, Crofton, MD 21114-4544
(410) 286-0092 · (410) 286-0093 FAX · (703) 927-9090

EDUCATION
EMPLOYMENT
ACADEMIC APPOINTMENTS
RESEARCH EXPERIENCE
CONSULTING EXPERIENCE
REGULATORY SCIENCE EXPERIENCE
INVITED PRESENTATIONS
SPECIALIZED TRAINING AND WORKSHOPS
RELEVANT PUBLICATIONS
PROFESSIONAL PRESENTATIONS
GRANTS HONORS AND AWARDS
ADDITIONAL SCIENTIFIC ACTIVITIES
PROFESSIONAL AFFILIATIONS
CRIMINAL/PATERNITY COURT CASES

EDUCATION:

B.S. Department of Biology, James Madison University, Harrisonburg, VA, 1976.
M.S. Department of Entomology, University of Maryland, College Park, MD, 1980.
Ph.D. Department of Microbiology, University of Maryland, College Park, MD, 1985.
Postdoctoral Fellow - Plant Protection Institute, U.S.D.A., Beltsville, MD, 1987.

EMPLOYMENT:

1. Founder/Consultant - DNA Diagnostics, Inc., P.O. Box 4544, Crofton, MD. February, 1993-Present.
2. Division Director - U.S. Environmental Protection Agency, Washington, DC. November, 2000-Present.
3. Associate Division Director ( Acting ) - U.S. Environmental Protection Agency, Washington, DC., October, 1999-June, 2000.
4. Branch Chief-U.S. Environmental Protection Agency, Washington, DC. February, 1997-November, 2000.
5. Team Leader/Microbiologist-U.S. Environmental Protection Agency, Washington, DC. August, 1989-February, 1997.
6. Legislative Assistant/Science Advisor-U.S. House of Representatives, Committee on Agriculture, Washington, DC. January, 1994-June, 1995.
7. Group Leader/Senior Staff Scientist-Digene Diagnostics, Inc., Silver Spring, MD. January, 1988-August, 1989.
8. Postdoctoral Fellow-U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD. January, 1985-December, 1987.
9. Research Assistant-The Johns Hopkins University School of Medicine, Department of Pharmacology and Experimental Therapeutics, Baltimore, MD. September, 1980-September, 1981.
10. Graduate Research Assistant-University of Maryland, Department of Entomology, College Park, MD. August, 1977-August, 1980.

ACADEMIC APPOINTMENTS:

1. Professor-George Mason University, Department of Molecular and Microbiology, Fairfax, VA. January, 1999-Present. An undergraduate level course in Microbiology, an undergraduate level course in Genetics, a graduate level methods and applications course in Forensic DNA Profiling, and a graduate level course in Forensic Sciences.
2. Assistant Professor/Adjunct Faculty-Northern Virginia Community College, Science and Applied Technologies Division, Alexandria, VA. August, 1996-December, 1997. Introductory course in Biology.
3. Assistant Professor/Adjunct Faculty-Anne Arundel Community College, Life Sciences Division, Arnold, MD. January, 1994-May, 1996. Introductory courses in Microbiology and Biology.
4. Instructor-EXON-INTRON, Inc., York, PA. January, 1996-Present. Responsibilities include teaching lecture and laboratory courses entitled "RFLP Technologies: Forensic and Research Applications," "DNA Typing: RFLP and PCR-Based Systems," and "PCR Methodology-RFLP/D1S80 Typing."
5. Assistant Professor/Adjunct Faculty-Montgomery College, Department of Science, Mathematics, and Applied Technologies, Germantown, MD. January, 1993-December, 1994. Introductory courses in Biology and Genetics.
6. Instructor-University of Maryland Baltimore County, Department of Biological Sciences, Catonsville, MD. September, 1981-September, 1984. Introductory course in Microbiology and an upper level course in Molecular Genetics.
7. Faculty Research Assistant-University of Maryland, Department of Microbiology, College Park, MD. May, 1982-January, 1985.

RESEARCH EXPERIENCE:

1. Group Leader/Senior Staff Scientist-Research involved the development and use of nonradioactive DNA probes for the detection and diagnosis of viral pathogens in clinical samples by in situ hybridization. Managerial responsibilities included administrative leadership, technical supervision, planning, developing, prepared and managed budget, administrating research activities, interfacing with all levels of management, and establishing research collaborations. Radiation Safety Officer, Biohazard Safety Officer, Principal Investigator/Project Manager for NIAID-supported SBIR grant, Principal Investigator/Project Director for NEI-supported SBIR grant. Dr. F. E. Taub, Digene Diagnostics, Inc., Silver Spring, MD.
2. Postdoctoral Fellow-Research involved the investigation of molecular events occurring during baculovirus replication in permissive, semipermissive, and nonpermissive cell lines. Additional projects included transcriptional and translational control mechanisms using baculovirus-infected cells as a model system, gene isolation and characterization, cloning, DNA restriction endonuclease mapping using various DNA/DNA hybridization techniques, sequencing, and investigating DNA-protein interactions. Dr. E. M. Dougherty, Insect Biocontrol Laboratory, U.S. Department of Agriculture, Beltsville, MD.
3. Research Assistant-Conducted research on the structure and function of the Epstein-Barr Virus genome. Using recombinant DNA technology a genomic library was constructed and used to establish a detailed restriction map for a region encoding the transformation function. Dr. S. D. Hayward, Department of Pharmacology and Experimental Therapeutics, The Johns Hopkins University School of Medicine, Baltimore, MD.
4. Graduate Research Assistant-Conducted research on the selection of a virulent strain of invertebrate viruses using chemical mutagenesis and serial passage. Dr. C. F. Reichelderfer, Department of Entomology, University of Maryland, College Park, MD.

CONSULTING EXPERIENCE:

1. Founder of DNA Diagnostics, Inc., P.O. Box 4544, Crofton, MD 21114-4544. February, 1993-present. A consulting firm specializing in the science, legal, and ethical issues surrounding the use of DNA technology in forensic science. Provide training in the science of DNA, interpretation of criminal and paternity cases where DNA or entomological analyses have been performed, and expert testimony.
2. Media Cybernetics, L.P., Silver Spring, MD 20910. 1995. Provided guidance on the usability of Gel-Pro Analyzer,^TM a Windows^TM-based software module which automates the collection and storage of data from electrophoretic gel images for forensic and gene linkage analyses of RFLP, VNTR, and PCR-based assays (HLA DQA1 and STRs).

REGULATORY SCIENCE EXPERIENCE:

1. Division Director - Provide day-to-day supervision and direction for the implementation and evaluation of all major science and science policy issues relating to hazard assessment and biotechnology. Serves as the principal point of contact and coordination point for interactions within EPA and with other Federal Agencies on activities such as biotechnology, children's health protection, and the Science Policy council. Also administers the activities of the Science Review Board and the Science Advisory Panel. Also, oversees the technical work of staff and ensures consistency of work products with applicable policies, precedents, and with the professional principles of the discipline involved. Mr. Joseph Merenda, Office of Science Coordination and Policy, U.S. Environmental Protection Agency, Washington, DC.
2. Associate Division Director (Acting) - Provide authoritative administrative and program expertise to the Director for all Division activities involving assessing the health and environmental hazards and risks of new and existing chemicals and microorganisms (naturally-occurring and genetically engineered) and preparing integrated hazard and risk assessment documents as needed to support the full range of OPPT's regulatory and non-regulatory programs. Involved in managing various aspects of U.S. participation in the Organization for Economic Cooperation and Development (OECD) Screening Information Data Sets (SIDS) program in high Production Volume Chemicals; reviewing and evaluating health and environment effects test data submitted under TSCA or other authorities administered by OPPT, and provided expert scientific support to Agency audits of testing programs producing such data. Responsible for developing and updating health and environmental hazards and risks of chemicals and microorganisms and ensuring that procedures and criteria employed by OPPT are consistent with Agency guidelines and the current scientific state-of-the-art. Provided authoritative administrative and procedural expertise for developing Division strategies, defining major program objectives, estimating required resource levels, reviewing and evaluating ongoing program activities and assessing work products. Also involved the Office of Research and Development in defining and reviewing Agency research related to characterizing the health and environmental effects of chemicals and biotechnology-derived products. Dr. Oscar Hernandez, Risk Assessment Division, Office of Pollution Prevention and Toxics, U.S. Environmental Protection Agency, Washington, DC.
3. Branch Chief-Provide supervision, leadership, and administrative direction to staff. Define major program objectives, estimate required resource levels, review and evaluate ongoing program activities and assess outputs produced. Provide support for developing and recommending program objectives and operations. Provide supervision to staff specialists to develop policies and objectives, allocating and managing resources, develop budget strategies and justifications, develop staffing patterns, and resolve operational problems. Also represent the Branch, Division or Office at various task force panel and committee meetings, involving development of management products, outreach, marketing and training activities. Exercise supervisory personnel management responsibilities over Branch members. Drs. Oscar Hernandez and Vanessa Vu, Risk Assessment Division, Office of Pollution Prevention and Toxics, U.S. Environmental Protection Agency, Washington, DC.
4. Team Leader/Microbiologist-Involved in providing strategies and establishing guidelines for assessing the risks associated with the introduction of transgenic pesticidal plants, biochemical pesticides, or products derived through biotechnology, into the environment. Also involved in the toxicological (both mammalian and ecological) evaluation of data pertaining to microbial and biochemical pest control agents, and in the evaluation of the molecular construction of genetically engineered microbial and transgenic plant pesticides for regulatory compliance under FIFRA/FFDCA/FQPA. Also involved in issues pertaining to international and domestic regulation of agricultural products derived using biotechnology. Additional tasks included the development of data requirements for transgenic plant testing, regulatory reform for biochemical pesticides, strategies for development/resolution of guidelines/policy issues related to products developed through biotechnology, and harmonization of regulatory requirements under the Canadian-United States Trade Agreement (CUSTA), NAFTA, and OECD. Dr. R. D. Sjoblad, Health Effects Division and Dr. J. Andersen, Biopesticides and Pollution Prevention Division, Office of Pesticide Programs, U.S. Environmental Protection Agency, Washington, DC.

INVITED PRESENTATIONS:

1. Scientist Faculty. DNA Cross-Examination College, 3rd Annual Forensic Science Conference, Public Defender Service for the District of Columbia, Washington, DC. September, 2005.
2. The ABCs of Forensic DNA Typing: STRs and Gender ID. Forensic Nursing: Forging Partnerships for the Future, 13th Annual Scientific Assembly of the International Association of Forensic Nurses, Arlington, VA September, 2005.
3. The Power of Forensic DNA Typing. The Department of Biology, James Madison University, Harrisonburg, VA. April, 2005.
4. The ABCs of Forensic DNA Typing. Fairfax Bar Association, Fairfax, VA. June, 2003.
5. Understanding Forensic DNA Evidence, Alexandria Bar Association, Continuing Legal Education course, Alexandria, VA. October, 2002.
6. DNA Typing as a Forensic Tool. Virginia College of Criminal Defense Attorneys and Virginia Public Defender Commission, Four For Forensics, Contiuning Legal Education course. University of Richmond, Richmond, VA. July 2002.
7. DNA Typing as a Forensic Tool. Public Defender's Office of the State of Delaware, Continuing Legal Education course. Wilmington, DE. June, 2001.
8. DNA Analysis. District of Columbia Bar Association, The Criminal Law and Individual Rights Section, Washington, DC, May, 2000.
9. The ABCs of DNA Typing: Principles and Methodologies. Office of Pollution Prevention and Toxics, Technical Training Committee, U.S. Environmental Protection Agency, Washington, DC. October, 1998.
10. Applications of DNA Technology in Forensic Science. Law Offices of Keefer, O'Reilly, Ferrario and Lubbers. Las Vegas, NV. August, 1996.
11. The ABCs of DNA: An Introduction to the Science of DNA. State of Maryland, Office of the Public Defender, Annual Conference, Oakland, MD. May, 1996.
12. Advanced Formula DNA: Crime Scene to Lab to Court. State of Maryland, Office of the Public Defender, Annual Conference, Oakland, MD. May, 1996.
13. DNA Technology in Forensic Science: A Prospective from the Defense. DNA 101-Unraveling the Mystery. Amer. Prosecutors Res. Institute, Criminal Prosecution Div., Alexandria, VA. November, 1995.
14. Forensic Applications of DNA Technology. DNA Working Group, Office of the Public Defender, State of Maryland, Ellicott City, MD. October, 1993.
15. The Use of DNA Technology in Forensic Science. Office of the Federal Public Defender, Baltimore, MD. April, 1995.
16. Applications of DNA Technology in Forensic Science. DNA Working Group, Office of the Public Defender, State of Maryland, Annapolis, MD. December, 1993.

SPECIALIZED TRAINING AND WORKSHOPS:

1. DNA Forensics, Profiling PCR and Beyond , Cambridge Healthtech Institute's 5^th Anniversary Meeting, Washington, DC. 2002.
2. Microarray Methods and Analysis Workshop. Institute for Biosciences, Bioinformatics and Biotechnology, George Mason Univ., Manassas, VA. 1999.
3. New DNA Technology: Approaches to Toxicology, Medical Diagnostics, Analytical Chemistry and Agricultural Testing. Genetic Toxicology Assoc., Newark, DE. 1999.
4. Resolution and Interpretation of Mixtures. DNA Forensic Analysis: New Trends and Technologies. IBC's Internat. Conf., Annapolis, MD. 1998.
5. PCR Qualification Strategies and Applications: New Developments of Real Time Detection. Perkin Elmer Applied Biosystems, National Institutes of Health, Bethesda, MD. 1998.
6. Advanced HPLC Detection for Analysis of Biomolecules. Millipore Corp., Waters Chromatography Div., Catonsville, MD. 1996.
7. Polymerase Chain Reaction (PCR) Applications/Cycle DNA Sequencing Workshop. American Type Tissue Collection, Rockville, MD. 1995.
8. Differential Display Polymerase Chain Reaction Workshop. EXON-INTRON, Inc., Columbia, MD. 1995.
9. Polymerase Chain Reaction Methodology Workshop. EXON-INTRON, Inc., Columbia, MD. 1993.
10. Sequence Specificity in Transcription and Translation Workshop. Cetus-UCLA Symposium. Steamboat Springs, CO. 1985.
11. Microscopy Training Course. Nikon Instrument Division, Garden City, NY. 1977.

RELEVANT PUBLICATIONS:

1. McClintock, J. T. PCR-based detection systems: Post transfusion and amelogenin typing. A case report. 2002. Abstract, Cambridge Healthtech Institute's 5th Annu. Meeting, DNA Forensics, Profiling, PCR and Beyond, Washington, DC.
2. McClintock, J. T. 2001. The power of DNA typing evidence. The District of Columbia Bar Newsletter. No. 1, September, 2001.
3. McClintock, J. T. 1999. DNA typing evidence in forensic casework. Fairfax Bar J. Vol. 26, No. 3.
4. McClintock, J. T., I-J Chan, S. R. Thaker, A. Katial, F. E. Taub, A. E. Aotaki-Keen, and L. M. Hjelmeland. 1992. Detection of c-sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization. In Vitro Cell. Develop. Biol. 28A: 102-108.
5. McClintock, J. T., M. Mosher, S. R. Thaker, W. K. Wacker, D. Jones, M. Forman, S. P. Adler, P. Charache, and F. E. Taub. 1991. Culture confirmation of cytomegalovirus and herpes simplex virus by direct enzyme-labeled DNA probes and in situ hybridization. J. Virol. Methods 35:81-91.
6. McClintock, J. T., D. Guzo, K. P. Guthrie, and E. M. Dougherty. 1991. DNA-binding proteins of baculovirus-infected cells during permissive and semipermissive replication. Virus Res. 20:133-145.
7. McClintock, J. T., I-J Chan, F. E. Taub, L. Resnick, and A. E. Friedman-Kien. 1991. Rapid detection of Epstein-Barr virus DNA in clinical samples of oral hairy leukoplakia with HRP-labeled DNA probes and in situ hybridization. J. Virol. Methods 33:155-164.
8. McClintock, J. T., R. D. Sjoblad, and R. Engler. 1991. Are genetically engineered pesticides different? Potential EPA data requirements for toxicological evaluation. Chemtech 21:490-494.
9. McClintock, J. T., D. Guzo, K. P. Guthrie, and E. M. Dougherty. 1991. DNA-binding proteins in baculovirus-infected insect cells. Abstract, 91st Annu. Meeting, Amer. Soc. Microbiol., Dallas, TX.
10. McClintock, J. T., S. R. Thaker, M. Mosher, D. Jones, M. Forman, P. Charache, K. Wright, J. Keiser, and F. E. Taub. 1989. Comparison of in situ hybridization and monoclonal antibodies for the early detection of cytomegalovirus. J. Clin. Microbiol. 27:1554-1559.
11. McClintock, J. T., A. Friedman-Kien, and F. E. Taub. 1989. Direct detection of Epstein-Barr virus in tongue tissue smears from individuals with suspected hairy leukoplakia. Abstract, 89th Annu. Meeting, Amer. Soc. for Microbiol., New Orleans, LA.
12. Wacker, W. K., J. T. McClintock, S. Strohofer, S. Khan, R. P. Baughman, and F. E. Taub. 1989. Direct detection of cytomegalovirus in bronchoalveolar lavages using HRP-labeled DNA probes and in situ hybridization. Abstract, 89th Annu. Meeting, Amer. Soc. for Microbiol., New Orleans, LA.
13. McClintock, J. T., and E. M. Dougherty. 1988. Restriction mapping of Lymantria dispar nuclear polyhedrosis virus DNA: Localization of the polyhedrin gene and identification of four homologous regions. J. Gen. Virol. 69:2303-2312.
14. Mosher, M., A. Maters, D. Jones, M. Forman, S. Grillo, J. T. McClintock, and F. Taub. 1988. Rapid detection of cytomegalovirus-infected cells by in situ hybridization with enzyme-linked DNA probes. Abstract, 88th Annu. Meeting, Amer. Soc. for Microbiol., Miami Beach, FL.
15. McClintock, J. T., and E. M. Dougherty. 1987. Superinfection of baculovirus-infected gypsy moth cells with the nuclear polyhedrosis viruses of Autographa californica and Lymantria dispar. Virus Res. 7:351-364.
16. McClintock, J. T., E. M. Dougherty, and R. M. Weiner. 1986. Protein synthesis in gypsy moth cells infected with a nuclear polyhedrosis virus of Lymantria dispar. Virus Res. 5:307-322.
17. Dougherty, E. M., B. J. Taylor, and J. T. McClintock. 1986. 5-Methylcytosine inhibition of a nuclear polyhedrosis virus of Autographa californica (AcMNPV). Abstract, 86th Annu. Meeting, Amer. Soc. for Microbiol., Washington, DC.
18. McClintock, J. T., E. M. Dougherty, and R. M. Weiner. 1986. Semipermissive replication of a nuclear polyhedrosis virus of Autographa californica in a gypsy moth cell line. J. Virol. 57:197-204.
19. McClintock, J. T. 1985. Baculovirus replication: Characterization of DNA and proteins synthesized by a nuclear polyhedrosis virus of Lymantria dispar, the gypsy moth, in a homologous cell line. Ph.D. Dissertation, Univ. of MD., College Park, MD.

PROFESSIONAL PRESENTATIONS:

1. McClintock, J. T., and E. M. Dougherty. 1987. Cosmid cloning and restriction endonuclease mapping of the DNA of the nuclear polyhedrosis virus of Lymantria dispar, the gypsy moth. Abstract, VIIth Internat. Congress Virol., Edmonton, Canada.
2. McClintock, J. T. 1985. DNA replication and protein synthesis during baculovirus replication. Abstract, Graduate Student Symposium, Washington Branch, Amer. Soc. of Microbiol., Fairfax, VA.

GRANTS HONORS AND AWARDS:

1. Travel Grant from the Agricultural Research Institute to attend the 91st Annu. Meeting, Eastern Branch, Entomol. Soc. Amer., Richmond, VA. $400. 1991.
2. Travel Grant from the Agricultural Research Institute to attend the 91st Annu. Meeting, Amer. Soc. Microbiol., Dallas, TX. $1200. 1991.
3. Citizen Ambassador Program, Virology Delegation to the People's Republic of China. A program of People to People Internat. Spokane, WA. 1991.
4. Principal Investigator for grant funded by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health entitled "Herpesviridae Infections and the Progression of AIDS." $50,000. 1989.
5. Principal Investigator for grant funded by the National Eye Institute of the National Institutes of Health entitled "PDGF Expression in Proliferative Retinopathies." $48,000. 1988.
6. Travel Grant from the Amer. Soc. for Virol. (sponsored by NIH) to attend the VIIth Internat. Congress Virol., Edmonton, Canada. $500. 1987.

ADDITIONAL SCIENTIFIC ACTIVITIES:

1. Consultant and Expert Witness-Review and data analysis of DNA tests results (e.g., RFLP analysis, PCR-based amplification and typing assays [e.g., HLA DQA1/ PM, STRs, D1S80, and gender (amelogenin) identification] and DNA hybridization techniques; court qualified) and entomological interpretations of forensic samples (court qualified) collected at crime scenes. Provide prosecutors, defense attorneys, as well as law enforcement officials with a basic understanding of the science of DNA and a thorough background of the molecular techniques used in DNA analyses, courtroom strategies, and effective presentation of DNA evidence at trial.
2. Scientific Program Committee and Conference Organizing Committee for the Annual Conference on Antimicrobial Resistance, 2004 and 2003. National Foundation for Infectious Diseases, Bethesda, MD.
3. Reviewer for the National Research Initiative Competitive Grants Program. 2000. U.S Department of Agriculture, Washington, DC.
4. Panelist of the U.S. Department of Agriculture Biotechnology Risk Assessment Research Grants Program. 2002, 2000, 1996, and 1995.  U.S. Department of Agriculture, Agriculture Research Service, Washington, DC.
5. Reviewer for refereed scientific journals such as Virus Research, Canadian J. of Microbiol., and J. of Agriculture and Food Chemistry.

PROFESSIONAL AFFILIATIONS:

• American Association for the Advancement of Science
• American Society for Microbiology
• American Society for Virology
• Genetic Toxicology Association

CRIMINAL/PATERNITY COURT CASES: